ExKine™ Nuclear and Cytoplasmic Protein Extraction Kit || Abbkine

Abbkine ExKine™ Nuclear and Cytoplasmic Protein Extraction Kit contains Cytoplasmic Solution A (CES A), Cytoplasmic Solution B (CES B), Nuclear Extraction Solution (NES), Protease Inhibitor (100X), DTT (500X).

Abbkine recently launches a series of Protein Extraction & Quantification Kits, see below listing, more detailed informaton, please click Sampling Kit.

Product Name Cat. No. Sizes

Protein Quantification Kit (BCA Assay)

KTD3001 500T/2000T/5000T

Protein Quantification Kit (Bradford Assay

KTD3002 500T/2000T/5000T
ExKine™ Nuclear and Cytoplasmic Protein Extraction Kit KTP3001 50T/200T
ExKine™ Nuclear Protein Extraction Kit KTP3002 50T/200T
ExKine™ Cytoplasmic Protein Extraction Kit KTP3003 50T/200T
ExKine™ Total Membrane Protein Extraction Kit KTP3004 50T/200T
ExKine™ Membrane and Cytoplasmic Protein Extraction Kit KTP3005 50T/200T
Protease Inhibitor Cocktail (100X) BMP1001 1ml/1ml×5

Today I will introduce ExKine™ Nuclear and Cytoplasmic Protein Extraction Kit to you.

Abbkine ExKine™ Nuclear and Cytoplasmic Protein Extraction Kit enable stepwise separation and preparation of crude cytoplasmic and nuclear extracts from mammalian cultured cells or tissue. This Kit is based on allowing cells to swell with hypotonic buffer. And then the cells are disrupted, the cytoplasmic fraction is removed, and the nuclear proteins are released from the nuclei by a high salt buffer. Non-denatured, active proteins are purified in less than two hours.

Western blot of specific proteins fraction using Abbkine ExKine™ Nuclear and Cytoplasmic Protein Extraction Kit. Sample: 293T cells lysate. C: cytoplasmic extraction was analyzed using α-tubulin antibody (A01080). N: nuclear extraction was analyzed using α-tubulin antibody (A01080).

Components:

1. Cytoplasmic Solution A (CES A)
2. Cytoplasmic Solution B (CES B)
3. Nuclear Extraction Solution (NES)
4. Protease Inhibitor (100X)
5. DTT (500X)

Features:

1. Versatile: suitable for fresh mammalian cultured cells and tissues with little or no cross-contaminations.
2. Fast and convenient: non-denatured, active proteins are purified in less than two hours.
3. Compatible: apply in various downstream assays, including Western blotting, gel-shift assays, protein assays, reporter gene assays and enzyme activity assays.

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