Determination Method of Amino Acid Purity

1. The importance of determination of amino acid purity

The determination of amino acid purity has important application value in the field of biopharmaceuticals. It can help us understand the biological activity of drugs, predict the stability of drugs, and optimize the production process of drugs. In addition, the determination of amino acid purity is also an important link in the quality control of biopharmaceuticals.

2. Method for Determination of Amino Acid Purity

There are many methods for the determination of amino acid purity, the most commonly used of which is high performance liquid chromatography (HPLC). HPLC can accurately and quickly determine the purity of amino acids, but requires specialized equipment and operating techniques. In addition, there are other methods, such as amino acid analyzer, gas chromatography and so on.

3. High performance liquid chromatography (HPLC)

HPLC is a method for the separation and quantitative analysis of amino acids. Its principle is to make use of the different solubility of amino acids in different solvents, and to separate amino acids in chromatographic columns by changing the ratio of solvents. Then, the purity of each amino acid is determined by a detector.

4. Amino acid analyzer

Amino acid analyzer is a kind of equipment specially used to determine the purity of amino acid. Its principle is to use electrochemical detectors to determine the purity of amino acids by measuring the electrochemical reactions of amino acids.

5. Gas chromatography

Gas chromatography is a method that uses the volatility of amino acids, volatilizes amino acids by heating, then separates them through a chromatographic column, and finally uses a detector to measure the content of amino acids.

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