New York, US – May 8, 2019 – Creative Biolabs, a prominent custom service provider in antibody production and engineering, recently improved its antibody humanization service. The company adopts three strategies to humanize antibodies, including CDR grafting and SDR grafting, chain shuffling, as well as humanized IgG library screening, so as to supply the best humanized antibodies of high specificity and affinity with minimized immunogenicity in humans.
Antibody humanization is of paramount importance to ameliorate or eliminate the immunogenicity of monoclonal antibodies derived from xenogeneic species and simultaneously to enhance their activation of the human immune system. The optimization of this service by Creative Biolabs is exactly intended to facilitate the antibody humanization process.
“We make great improvements to our antibody humanization service by optimizing three strategies. First, CDR grafting and SDR grafting. Six CDR loops comprising the antigen-binding site are grafted into corresponding human framework regions. Then, computer modeling will be used to randomize certain framework residues. The grafted CDRs together with the randomized residues are cloned into a phage display library, the screening of which then helps select the humanized antibodies with the best affinity. Afterward, SDR grafting will be adopted to reduce the immunogenicity of CDR-grafted humanized antibodies,” said a scientist from Creative Biolabs.
“Second, chain shuffling. In this approach, the light chain of the rodent antibody is replaced by light chains from one of the human antibody libraries. The resulting hybrid antibody library is then screened against the particular antigen. Thereafter, the heavy chain of the selected hybrid antibodies gets replaced by heavy chains of the human antibody library. Eventually, the subsequent screening of this secondary chimeric library will generate fully humanized antibodies, which have higher affinity than the original antibodies. Importantly, with this method, we can produce the 100% humanized mouse antibody.”
“In the third strategy, humanized IgG library screening, a mammalian cell surface display library is established to display full-size humanized IgG variants for further selection. This method allows the selection of humanized antibodies in a full-size IgG format that retain or increase the original affinity of the mouse antibody. Over the past decade, our company has performed 15 murine humanization projects; at least one humanized antibody has entered the clinical trials. By the way, we are the only company in the industry with the expertise in the NHP antibody, especially the humanized monkey antibody.”
In conclusion, this optimized service from Creative Biolabs marks a great jump forward for the antibody humanization field. Each of the three humanization strategies elaborated above has its unique advantages and is expected to satisfy the demanding requirements of the global customers. With the advancement of biological science and technology, these strategies will be further upgraded to produce the desired humanized antibodies of higher quality.
About Creative Biolabs
Creative Biolabs is a leading custom service provider, boasting rich experience in antibody production and engineering. Its service portfolio includes mouse and rat monoclonal antibody production using hybridoma technology, human, monkey, rabbit, chicken, dog, llama and camel monoclonal antibody production using various antibody library technologies. In addition, the company also conducts in-depth antibody humanization and affinity maturation.
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Company Name: Creative Biolabs
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Phone: 1-631-830-6441
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State: New York
Country: United States
Website: www.creativebiolabs.com