Recent advances in molecular biology have improved the detection applications of bioluminescence and chemiluminescence. These developments facilitate the continuous detection of biological processes in vitro and in vivo and can be applied to clinical, diagnostic and drug development. Moreover, hypersensitive and selective bioanalytical tools, such as recombinant cell biosensors, immunoassays and nucleic acid hybridization systems, have been developed by combining chemiluminescent enzyme assays or certain photoluminescent proteins with biologically specific binding sites at the gene level.
Since more than 20 years ago, Marlene DeLuca’s first successful access to the transgenic tobacco of firefly fluorometric enzyme assays, the application of bioluminescence and enzymology assays have entered a new era.
New BL / CL biotechnology tools
Reported gene technology represents one of the recent major advances in molecular biology. The reporter gene is a DNA sequence that encodes a protein or enzyme that is easily detectable. The genes can be artificially introduced into a cell to monitor the expression of the gene to obtain the effect of whole cell biosensor or cell localization. The application of reporter gene technology has been applied to the development of BL / CL whole cell biosensors.
To the direction of miniaturization analysis
Miniaturization analysis equipment, such as micro-matrix and microfluidic devices, has been fully developed, while different approaches based on traditional small-scale analysis have also been further developed, such as microplates.
BL / CL imaging
Low-light imaging equipment is based on ultra-high sensitivity CCD camera that can be detected by the sample surface after a specific reaction to emit light. These devices not only can quantify the intensity of light at the single molecule level, but also can position.
BL / CL biosensor
BL / CL is used as a detection system in a biosensor, meaning that it is used as an analytical device in a biometrics system and in contact with a conductive device, and the associated enzymes are immobilized on a variety of different forms of devices. These systems are mainly based on the coupling of the enzymatic reaction and trigger the emission of photons.
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Company Name: Creative Enzymes
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Website: http://www.creative-enzymes.com