New York, June 01, 2018 – Proteomics, as a basic biological challenge, based on high-resolution mass spectrometry (MS) has evolved into an efficient tool for the analysis of thousands of proteins. Any large-scale proteomics-related research needs the ability to reliably identify as many protein components as possible. Currently, the sensitivity of mass spectrometers on the market allows scientists to study the entire proteome in depth, detect protein expression patterns, and ultimately identify and characterize protein behavior under specific environmental, physiological, or disease conditions. Therefore, the application of proteomics is diverse, including the identification of clinical biomarkers in molecular diagnostics, the understanding of drug resistance and the large-scale analysis of post-translational modifications (PTM), even de novo peptides sequencing are included..
At present, a new revolutionary method introduced through the timsTOFTM Pro mass spectrometer, that is, the Parallel Accumulative Continuous Fragmentation (PASEF), aiming to offer a more powerful proteome analysis method to help researchers discover low-level, biologically significant proteins and validate them in research.
MS-based techniques can perform large-scale, systematic measurements on proteomics and generate biological insights from the computational analysis of proteomics data sets, and these methods allow accurate identification and accurate quantification of almost all proteins that have been expressed. Currently, there are two main methods for the study of proteins in MS, that are, top-down proteomics and bottom-up proteomics, in which, top-down proteomics has the advantage of measuring any post-translational modifications (PTM), making the protein form well-characterized. However, implementing high-throughput top-down proteomics is challenging for many laboratories. While bottom-up proteomics includes the extraction of proteins from raw materials, and then separated with liquid chromatography (LC) and subsequently analyzed with MS.
PASEF, as an emerging method, that can store several precursor ions in parallel in a trapped ion transport device and then perform tandem fragmentation. It can implement hundreds of MS/MS events with the highest sensitivity, and increased DDA rates. PASEF technology can be specifically applied to the TIMS quadrupole time-of-flight mass spectrometer (QTOF), which adds an additional separation dimension to the standard MS scan. In addition to the application in interactive proteomics and PTM-related proteomics, PASEF’s analysis of protein mixtures from patient samples in clinical proteomics studies can be applied to inform the diagnosis and treatment of many diseases.
MS-based proteomics provides important insights into the composition, regulation, and function of molecular pathways. MS-based proteomics will continue to have a positive impact on biology and medicine by accurately identifying and quantifying thousands of proteins from complex samples. In the future, PASEF may be able to further expand the field of personalized medicine, which may enable clinicians to divide patients into drug-resistant and non-resistance groups, thereby promoting the application of targeted therapies.
About Creative Proteomics
Creative Proteomics is professional in offering diverse proteome-related service, including post-translational modifications, top-down proteomics, bottom-up proteomics, protein identification etc. Besides, we can also offer the custom program for your study per the specific requirements.
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