New York – Apr 2nd, 2018 – Creative Biogene, as a professional biotechnology organization, has established a large number of reporter stable cell lines suitable for multiple applications. In the studies of life science and biotechnology, the commonly used reporter genes are visually identifiable including green fluorescent protein (GFP), red fluorescent protein (RFP), luciferase, secreted alkaline phosphatase (SEAP) etc. This new report will focus on sharing the application of GFP offered by Creative Biogene.
GFP has been considered as a marker for gene expression and protein targeting in intact cells. In biological research, it is widely used as a genetically encoded fluorescent marker. This fluorescent label enables multicolor labeling and is used to study protein-protein interactions. GFP and its derivatives with different colors can be used for a variety of applications, such as studying the function and organization of living systems in protein tags, examining gene expression and various biological options. GFP provides researchers with a rich palette of different spectral and biochemical characteristics of derivatives.
The Application in Cell biology and biotechnology
Since the advent of GFP (autofluorescent protein), whole living cells or organisms have been used as experimental systems in a wide range of fields (cell biology and biomedical). Since 1994, GFP has served as a scientist’s agent in the field of biotechnology because it only requires oxygen and energy to function. Fluorescent proteins have become the first line of investigation in the infinite area. It is used in various applications of biotechnology, such as protein fusion, imaging whole organisms, and transcription reporters.
The Application of Bacterial Protein Mapping
As a reporter for DNA and protein localization, GFP provides a highly sensitive and innovative method to study bacterial cell tissue, which has led to a new understanding of various cellular processes. GFP involves several features that can be used for local bacterial studies. As an alternative to immunofluorescence microscopy, GFP gene expression is used to examine major cell functions such as DNA replication, protein translation, and signal transduction. DNA and protein localization can now be monitored with GFP method to reveal the essential proteins involved in spore formation and cell division and development that are dynamically identified in the cell cycle. The utilization of GFP to label multiple components within a single cell and monitor the persistence of genetic material makes this method a useful tool for solving the complex uncertainties associated with bacteria.
The Application in Host-pathogen Interaction Studies
GFP has potential applications in the study of bacterial pathogenesis, that can be used to study the host-parasite interactions among three bacterial pathogens – Salmonella typhimurium, Y. pseudotuberculosis and M. tuberculosis. At present, the application of GFP in the study of host pathogens has been extensively successful because it is a low-toxic cytoplasmic protein that can be synthesized continuously, thus reducing the dilution effect of fluorescent signals during bacterial replication, and is easy to image and calculate the relationship and organization with living cells. Furthermore, it can be used to understand the colonization, proliferation, toughness, and proliferation of pathogens in living animals as a biological tracer.
The Application as A Reporter Gene
GFP is considered to be the most important and powerful tool for monitoring gene expression in different kinds of cells. It can be used as a reporter gene as it does not require the addition of any additional substrate. Meanwhile, GFP was used to measure mec-7 gene expression in the nematode Caenorhabditis elegans in vivo. If presenting a promising method to monitor the efficiency of gene transfer in transgenic animals and embryos.
The Application as A Fusion Tag
The most prosperous application of GFP is to regard it as a genetic fusion partner of host proteins in order to observe their localization and fate. The GFP gene is fused to the gene encoding the protein of interest and expressed in the cell. Incorporation of GFP can be accomplished at the amino- or carboxyl-terminus of the protein. The fusion protein retains its normal function and the fluorescent properties obtained through the GFP expression gene. All major organelles such as cell membranes, Golgi, nuclear and endoplasmic reticulum become targets via GFP.
The Prospect of GFP Application
GFP can be used to visualize specific cell types in intact animals, organs and tissues, which will play a significant role in the fields of immunology, neurobiology, development and the onset of cancer. The discovery of several color variants of GFP mutants has promoted the continued development of fluorescence instruments for cell imaging and detection. The stimulating innovations of novel green fluorescent proteins from other species provide GFP with bright future applications in cell, molecular and drug development.
About Reporter Stable Cell Lines Offered by Creative Biogene:
Based on years of experience and in-depth investigation, scientists at Creative Biogene have established a large number of stable reporter cells in many popular cell lines suitable for multiple applications.
Constitutive Reporter Cell Lines –
– GFP Reporter Cell Lines
– RFP Reporter Cell Lines
– GFP/RFP Reporter Cell Lines
– Dual Reporter Cell Lines
– Inducible Reporter Cell Lines
Company Name: Creative Biogene
Contact Person: Wendy Wilson
Email: Send Email
Address:45-1 Ramsey Road
State: New York
Country: United States