How to choose the right loading control antibodies for sample species?

Abbkine offers β-actin, Tubulin, GAPDH, Lamin B1, PCNA, Na+/K+-ATPase loading control antibodies for your sample tissues and cells of mammals and Plant actin and Rubisco loading control antibodies for your plant samples.

There are many kinds of loading control antibodies on the market, how to choose the right one for your own experiment based on sample species?

For tissues and cells of mammals, it is generally recommended to choose the proteins like β-actin, Tubulin, GAPDH, Lamin B1, PCNA, Na+/K+-ATPase, etc. Plant actin and Rubisco are often used in plant samples.

1. If you extract total proteins or cytoplasmic proteins from cells, β-actin, GAPDH and Tubulin will be your choice

Product Name MW Catalog No. Applications
Anti-β-Actin Mouse Mab (1C7) 43 kDa A01010 WB, IHC, IF
Anti-GAPDH Mouse Mab (2B5)  37 kDa A01020 WB, IHC, IF
Anti-β-Tubulin Mouse Mab (3G6)  55 kDa A01030 WB, IHC, IF
Anti-α-Tubulin Mab (3G5)  55 kDa A01080 WB, IHC, IF, IP
Anti-Cyclophilin B Mab (7B2) 24 kDa A01130 WB, IHC, IF
Cofilin Polyclonal Antibody 19 kDa ABP51018 WB, IHC, IF, ELISA
Vinculin Polyclonal Antibody 116 kDa ABP52701 WB, IHC, IF, ELISA

2. For the detection of mitochondrial proteins, you can use the following loading controls

Product Name MW Catalog No. Applications
Anti-COX IV Mouse Mab (14Y2) 16 kDa A01060 WB, IHC, IF
HSP60 Rabbit Polyclonal Antibody 60 kDa ABP51585 WB, IHC, IF
VDAC1 Rabbit Polyclonal Antibody 31 kDa ABP53121 WB

3. If your protein of interest is expressed in the nucleus, Histone H3 and PCNA are often selected by researchers

Product Name MW Catalog No. Applications
Anti-PCNA Mouse Mab (1D7) 29 kDa A01040 WB, IHC, IF
Anti-Histone H3 Mouse Mab (2D10) 15 kDa A01070 WB, IHC, IF, IP
Anti-Lamin B1 Monoclonal Antibody (15T1) 66 kDa A01090 WB, IHC, IF, IP
Anti-TBP/TATA Binding Protein Mab (2C6) 38 kDa A01120 WB

4. For membrane proteins, the commonly used protein is Na+/K+-ATPase; and you can use transferrin for serum samples

Product Name MW Catalog No. Applications
Na+/K+-ATPase α1 Rabbit Pab 113 kDa ABP51894 WB, IHC, IF
Transferrin Rabbit Polyclonal Antibody 77 kDa ABP52968 WB, IHC

5. Plant Actin and Rubisco are the representatives of loading controls for plant samples

Species Product name Catalog No. Applications
Plants Anti-Plant Actin Mouse Mab (3T3) A01050 WB
Plant-actin Rabbit Pab ABP50153 WB
Anti-Rubisco (Large Chain) Mab (9Y6) A01110 WB

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