New York – Jun 29th, 2018 – Creative Biogene, world’s top biotechnology product and services offer since 2005, providing the widely used tools in biology research – gene overexpression service by introducing new functions and applications, including GPCR stable cell lines, Ion channel stable cell lines and Reporter stable cell lines. Besides, the professional recombinant stable cell lines we generate can also express other popular gene targets such as PDEs, protein kinases etc. With this professional service offered, Creative Biogene will save time and money with a fast turnaround time and competitive price for related research.
In the parallel trends occurring in other areas of genetics, over-expression research has entered a new phase. Although targeted overexpression of individual genes provides valuable information, random screening will continue to be a powerful tool with clear advantages, systematic query genomic approaches may dominate over the next decade. Pioneer system screening has been performed using a large but incomplete overexpression source in yeast, flies, plants and human tissue culture systems. The challenge for the future will be to complete resource collection and develop high-throughput screening technologies to promote their use. Based on the initial systematic overexpression screening studies in yeast, we can expect to over-express interaction networks to provide new genetic linkages because the results are aggregated with other large data, such as physical interactions and deletion collection results.
What we can learn from systematic knockout studies is that individual loss-of-function mutations are not sufficient to extrapolate gene function. If more genetic methods are needed, it will be difficult to change the direction of research. Overexpression studies certainly have a rich history, establishing functional links for essentially any cellular process in several species, which offers offer several advantages as follows:
(1) A multifunctional tool that can be applied in a variety of ways in wild-type and mutant backgrounds;
(2) It can determine the regulatory rate limiting step;
(3) It has a dominant effect, so can be easily performed in diploid organisms;
(4) Provide functional links even for redundant genes;
(5) It determines the complementary interaction of the loss of function screen.
On the other hand, there are at least two obstacles that prevent wider use of overexpression. Targeted overexpression of individual genes can be performed in virtually any organism, but technical limitations and lack of adequate resources have inhibited routine whole genome overexpression screening. Biological limitations, such as the inability to preserve the introduced DNA as a plasmid, will remain in certain species, but the lack of resources is not an insurmountable challenge. The more difficult barrier to evaluation is the erroneous view that when genes are expressed on a non-physiological level, it is difficult to collect meaningful biological insights. However, the concerns of studying the overexpression phenotype are indistinguishable from the background of any other mutation, whether the pathway is knocked out, the dominant gains functional or overexpression damage, and the cells are disturbed. Although the potentially confusing new morphological effects are still possible, the experience and results of the first large-scale system screening show that they are not common and have been overwhelmingly balanced by providing a wealth of benefits. Most importantly, it is shown that when appropriate secondary screening criteria are used properly, overexpression can be as effective as any other screening technique, and informative and versatile.
About Creative Biogene
Founded in 2005, Creative Biogene provides consumers with kits, reagents, and services for biology-related research to accelerate the development of gene discovery, regulation, and function. Creative Biogene is a well-recognized industry leader for genomics and proteomics products and services with more than ten years’ rich experiences and million customers worldwide.
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