New York – Apr 30th, 2018 – Creative Bioarray, as an innovative biotechnology company, dedicated to offering worldwide customers with professional services and high-quality products to promote the development of biology-related research and progress of life science, of which, In situ hybridization, as part of histology services, can be applied in research, as well as clinical applications, especially for diagnostic purposes.
The main advantage of in situ hybridization offered by Creative Bioarray is that it allows researchers to determine how the distribution of a specific nucleic acid uses immunohistochemistry with the protein products of the target gene and their relationship to the cell structure. This method is currently an important tool in scientific research and clinical settings. The spatial information obtained from this method has greatly facilitated researchers’ understanding of many different areas of research such as viral infections, gene mapping, cytogenetics, gene expression, and prenatal diagnosis and development.
And for in situ hybridization, there will be several basic principle involved: the preparation of tissues (an important step to promote the entry of target nucleic acid), probe preparation (researchers must consider sensitivity and specificity, production equipment, ease of probe penetration through the tissue, stability of the hybrid, application, and reproducibility of the method while choosing in situ hybridization probes), as well as probe labeling and signal detection (including Radioisotope labeling and non-isotopic labeling).
One of the major advantages of in situ hybridization is that it makes the best use of difficult-to-obtain tissues (eg, embryos and clinical biopsies). Hundreds of different crosses can be made on the same tissue. The tissue bank can be formed and stored in a refrigerator for future use. However, the disadvantage of using in situ hybridization techniques is that it is difficult to identify targets with low copies of DNA and RNA. The sensitivity of in situ hybridization can be achieved through amplifying the target nucleic acid sequence prior to in situ hybridization or detecting the signals and developing new methods after the hybridization is completed.
Since the occurrence of in situ hybridization, many different methods have been developed. Some of the commonly used techniques including:
PCR using in situ hybridization (in situ PCR) amplifies a specific DNA or RNA sequence in a cell or tissue section, which is primarily used to identify DNA sequences that are not easily detectable using standard in situ hybridization. Primer in situ labeling is a rapid single-step target amplification method, which is another option for in situ PCR and fluorescence in situ hybridization (FISH) and has many potential diagnostic applications. The above techniques can be used to increase sensitivity before in situ hybridization.
For amplification, the signal after In situ hybridization, catalyzed reporter deposition (CARD) can increase the sensitivity of in situ hybridization signals from 500x to 1000x, and the specificity of probe hybridization will not be affected. Another method to amplify the signal is branched DNA technology. This technique improves the detection of nucleic acids by adding signals instead of amplifying the target or probe sequences.
Fluorescence in situ hybridization is an effective technique that allows direct observation of genetic changes in cells. This technique has many applications and is often applied to check for imbalances, gain or loss of fragments of chromosome material, or show specific breakpoints with or without imbalances. A common use of FISH in cytogenetic analysis is chromosomal gene mapping, characterization of genetic abnormalities, identification of genetic abnormalities associated with genetic diseases or neoplastic diseases, and detection of viral genomes in interphase nucleus or metaphase chromosomes.
Except for the above-mentioned technology, Creative Bioarray also offered Multicolor FISH (M-FISH) Analysis to support the in situ hybridization.
About Creative Bioarray
We focus on offering a wide range of high-quality cells, cell culture medium and reagents, FISH probes, tissue arrays, microorganisms and equipments. In addition, we also offer series of cell services, biosample services and histology services for the researchers to further develop the programs.
Creative Bioarray also uses the latest technology or proprietary protocols to validate and develop quality products that drive scientific innovation and standards.
Company Name: Creative Bioarray
Contact Person: Chelsea Clark
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Address:45-1 Ramsey Road Shirley
City: Long Island City
State: New York
Country: United States